| چکیده انگلیسی مقاله |
B ackground: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infec- tion in inbred ICR mice as a model of cerebral malaria (CM). M ethods: Infection in mice was initiated by intraperitoneal injection of 2 x 107 (0.2ml) parasitized red blood cells (PRBCs). R esults: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several i m- portant features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmo- nary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney’s medulla and cortex. Proinflammatory cytokines TNFα, IFNγ, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice. C onclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis. |
| نویسندگان مقاله |
r بصیر | r basir
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
ss fazalul رحیمیان | ss fazalul rahiman
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
k hasballah | k hasballah
department of pharmacology, faculty of medicine, syiah kuala university, 23111 darussalam, banda aceh, nanggroe aceh darussalam, indonesia.
wc chong | wc chong
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
h talib | h talib
department of pathology, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
mf یام | mf yam
pharmacology discipline, school of pharmaceutical sciences, universiti sains malaysia, 11800 penang, malaysia.
m jabbarzare | m jabbarzare
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
th tie | th tie
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
f احمد عثمان | f othman
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
مام moklas | mam moklas
department of human anatomy, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
wo عبدالله | wo abdullah
department of medical microbiology and parasitology, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
z احمد | z ahmad
department of pathology, faculty of medicine amp;amp; health sciences, universiti putra malaysia, 43400 serdang, selangor, malaysia.
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