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Iranian Journal of Parasitology، جلد ۷، شماره ۴، صفحات ۱۷-۲۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of Recombinant SAG1 Protein for Detection of Toxoplasma gondii Specific Immunoglobulin M by ELISA Test
چکیده انگلیسی مقاله B ackground: Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec- SAG1) for the detection of Toxoplasma -specific IgM antibodies in human sera, by an enzyme-linked immunosorbent assay (ELISA). M ethods : The purified recombinant protein SAG1 was applied in house ELISA test and the ability of it in binding to specific immunoglobulin M in 30 serum samples of acute infected patients was evaluated. The results obtained by assays with the recombinant SAG1 and standard commercial as- says were compared. R esult: The sensitivity and specificity of in house ELISA compared to a standard commercial ELISA (com-ELISA) were 80% and 90%, respectively. C onclusion: It was concluded that the rec-SAG1 could be an alternative marker for detection of anti Toxoplasma -specific IgM and diagnosis of acute infection.
کلیدواژه‌های انگلیسی مقاله Toxoplasma gondii,Recombinant SAG1,IgM,ELISA
نویسندگان مقاله n جلال لو | n jalallou
dept. of medical laboratory science, school of medicine, army medical science university, tehran, iran.


m بنده پور | m bandehpour
dept. of biotechnology, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

h خزان | h khazan
dept. of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

a حقیقی | a haghighi
dept. of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

b کاظمی | b kazemi
dept. of biotechnology, shahid beheshti university of medical sciences, tehran, iran and cellular and molecular biology research centers, shahid beheshti university, of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/279
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زبان مقاله منتشر شده en
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