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Iranian Journal of Parasitology، جلد ۸، شماره ۲، صفحات ۳۳۷-۳۴۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular identification of leishmania species using samples obtained from negative stained smears.
چکیده انگلیسی مقاله Background: Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcers sus-pected to CL by PCR method. Methods: Among 81 patients with suspicious skin lesions to CL referred to the Parasitology lab, negative Giemsa stained smears were collected. DNA extraction performed by scraping stained smears, then PCR was performed. Results: Among the DNA extracted from smears, L. tropica was isolated from 9 (11.1%) of the smears and L.major was not isolated from any samples. Conclusion: Direct microscopy on stained smears for diagnosis of leishmaniasis is not enough accu-rate. PCR is recommended for clinically suspected lesions with negative result of direct smear.
کلیدواژه‌های انگلیسی مقاله Cutaneous Leishmaniasis,Direct smear,Leishmania tropica,PCR
نویسندگان مقاله ma محقق | ma mohaghegh
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

a فتی | a fata
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran and cutaneous leishmaniasis research center, emam reza hospital, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

gh صالحی | gh salehi
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

f برنجی | f berenji
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

m موسوی بزاز | m mousavi bazzaz
department of community medicine, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

h رفعت پناه | h rafatpanah
inflammation and inflammatory diseases research center, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

m پریان | m parian
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

a موحدی | a movahedi
department of medical parasitology and mycology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/486
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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