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Iranian Journal of Parasitology، جلد ۸، شماره ۲، صفحات ۱۸۶-۱۸۹
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عنوان انگلیسی Axenic cultivation and pathogenic assays of acanthamoeba strains using physical parameters.
چکیده انگلیسی مقاله Background: The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. Methods: Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. Axenic cultivation was performed using Proteosepepton, yeast extract and glucose medium and TY-I-S33culture. Pathogenic survey was done using osmotolerance and thermotoler-ance assay. Briefly, differentosmolarity (0.5 M and 1 M) of non-nutrient agar plates were performed. One hundred fiftyμl of axenic culture were collected and were inoculated in 1% agar medium. For thermotolerance assay 150 μl of amoebas from axenic culture were divided into fresh culture me-diums. Cultures were incubated at 37oC and 42 oC. All plates were monitored for 24 h, 48 h and 72 h. Results: Overall, 16 strains of Acanthamoeba isolates previously genotyped as T4 were cultivated axenically after several months. Thermotolerance and osmotolerance assay revealed that all of clinical strains, soil and animal feces strains were highly pathogenic isolates. Two dust and water strains did not grow at high temperature (42 oC) and osmolarity (1.5 M) and thus they were classified as weak pathogens. Conclusion: Most of T4 genotypes are highly pathogenic organisms. This is an important finding since Acanthamoeba belonging to T4 type is the predominate genotype in environmental and clinical samples. The presence of highly pathogenic Acanthamoeba may pose a risk within susceptible people
کلیدواژه‌های انگلیسی مقاله Acanthamoeba,Axenic cultivation,Pathogenicity
نویسندگان مقاله m نیتی | m niyyati
department of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

h عابد خجسته | h abedkhojasteh
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran,iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی صالحی | m salehi
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran,iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

sh فرنیا | sh farnia
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran,iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد رضاییان | m rezaeian
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran,iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/507
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