این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۸، شماره ۳، صفحات ۴۰۸-۴۱۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Immunoproteomic Analysis of the Excretory-Secretory Proteins from Spirometra mansoni Sparganum.
چکیده انگلیسی مقاله Background: Sparganosis is caused by the invasion of Spirometra sparganum into various tissues/organs. Subcutaneous sparganosis can be diagnosed by biopsy , while visceral/cerebral sparganosis is not easy to be diagnosed. The diagnosis de-pends largely on the detection of specific anti-sparganum antibodies. T he specific-ity of the ELISA could be increased by using S. mansoni sparganum excretory– secretory (ES) antigens, but it also had the cross-reactions with sera of p atients with cysticercosis or paragonimiasis . The aim of this study was to identify early specific diagnostic antigens in S. mansoni sparganum ES proteins . Methods: The sparganum ES proteins were analyzed by two-dimensional electro-phoresis (2-DE) and Western blot probed with early sera from infected mice at 14 days post-infection. The immunoreactive protein spots were characterized by MALDI-TOF/ TOF-MS. Results: A total of approximately 149 proteins spots were detected with isoelectric point (pI) varying from 3 to 7.5 and molecular weight from 20 to 115 kDa and sev-en protein spots with molecular weight of 23-31 kDa were recognized by the infec-tion sera. Three of seven spots were successfully identified and characterized as the same S. mansoni protein (cysteine protease), and the proteins of other 4 spots were not included in the databases. Conclusion: The cysteine protease from S. mansoni ES proteins recognized by early infection sera might be the early diagnostic antigens for sparganosis.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله دان دان hu | dan dan hu
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.


جینگ cui | jing cui
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.


li ونگ | li wang
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.


li na liu | li na liu
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.


tong wei | tong wei
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.


zhong quan ونگ | zhong quan wang
dept. of parasitology, medical college, zhengzhou university, zhengzhou, p. r. china.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/476
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات