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Iranian Journal of Parasitology، جلد ۸، شماره ۳، صفحات ۳۸۲-۳۸۸
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عنوان انگلیسی Identification of leishmania species using PCR assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients.
چکیده انگلیسی مقاله Background: Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. Methods: In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasis that referred to health centers and infected during the epidemic of cutaneous leishmaniasis in Poldokhtar city, Lorestan Province, Iran in 2006.Genomic DNA from each slide was extracted. After DNA extraction, ITS-PCR was used. Results: Out of 178 slides, 129 (72.47%) samples had a band in the range of 485 bp and 49 (27.53%) samples 626 bp that matched L. tropica and L. major standard samples, respectively. Conclusion: This study showed that Leishmania DNA could be efficiently ex-tracted and amplified even from old Giemsa-stained microscopic slides that were stored more than 6 yr. In this study was shown that both L. tropica and L. major species exist in Lorestan Province.
کلیدواژه‌های انگلیسی مقاله Cutaneous leishmaniasis,Giemsa,Iran,Leishmania,PCR
نویسندگان مقاله فرناز خیراندیش | farnaz kheirandish
razi herbal medicines research center, department of parasitology and mycology, lorestan university of medical sciences, khorramabad, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

علی چگنی شرفی | ali chegeni sharafi
department of communicable disease control and prevention, deputy of health, lorestan university of medical sciences, khorramabad, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

بهرام کاظمی | bahram kazemi
cellular and molecular biology research center, shahid beheshti university, m.c., tehran, iran and department of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran and department of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی محبعلی | mehdi mohebali
department of parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

امان الله سرلک | amanollah sarlak
razi herbal medicines research center, department of parasitology and mycology, lorestan university of medical sciences, khorramabad, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

محمد جواد طراحی | mohamad javad tarahi
department of epidemiology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

کورش holakouee | kourosh holakouee
department of epidemiology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

هما حجاران | homa hajaran
department of parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/480
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