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Iranian Journal of Parasitology، جلد ۸، شماره ۳، صفحات ۳۷۶-۳۸۱
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عنوان انگلیسی Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran.
چکیده انگلیسی مقاله Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different re-striction enzymes of Taq 1, Rsa 1 and Alu 1 . Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy-sis also were the same for all isolates. PCR-RFLP patterns restriction en-zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu 1 bands were as follows: two approx-imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi-nant genotype in this province.
کلیدواژه‌های انگلیسی مقاله Hydatid cyst,ITS1,RFLP-PCR,Strain
نویسندگان مقاله هاله حنیفیان | haleh hanifian
dept. of medical parasitology and mycology, faculty of medicine, urmia university of medical sciences, urmia, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

کامبیز دیبا | kambiz diba
dept. of medical parasitology and mycology, faculty of medicine, urmia university of medical sciences, urmia, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

خسرو حضرتی تپه | khosrow hazrati tappeh
cellular and molecular research center, urmia university of medical seiences, urmia-iran.

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

حبیب محمدزاده | habib mohammadzadeh
dept. of medical parasitology and mycology, faculty of medicine, urmia university of medical sciences, urmia, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

رحیم محمودلو | rahim mahmoudlou
dept. of surgery, faculty of medicine, urmia university of medical sciences, urmia-iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/481
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