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Iranian Journal of Parasitology، جلد ۸، شماره ۳، صفحات ۳۶۷-۳۷۵
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عنوان انگلیسی In Vitro and In Vivo Potential of RH Strain of Toxoplasma gondii (Type I) in Tissue Cyst Forming.
چکیده انگلیسی مقاله Background: Based on recent studies, there are controversial reports on the ca-pacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capac-ity was evaluated by in vivo and in vitro experiments. Methods : RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathological and bioassay methods. The parasite was cultivated in the cell monolayer. To change to bradyzoite, the media pH was altered to 6.8. Biologi-cal aspect of the bradyzoites was evaluated by incubation in acidic pepsin and it's inoculation in ten BALB/c mice. Results : All rats showed antibodies to Toxoplasma at titers ≥1:320 but no DNA and tissue cyst were detected in the tissues. Following intraperitoneal inoculation of rats’ brain homogenate into BALB/c mice, no infection was established in none of the animals. During presence of cell culture, in acid media for a 3-5 days period, cyst-like structures were noticed when they were stained with PAS. The visible bradyzoites in the cysts that were incubated in acid pepsin medium were not able to kill any mice. Conclusion : This study confirmed that Iranian RH strain has lost the potential of tissue cyst forming in rats and bradyzoites cultivated in cell culture lost their resis-tance to acidic condition, so this strain can be a candidate for future vaccine re-searches.
کلیدواژه‌های انگلیسی مقاله RH strain,Tissue cyst,Toxoplasma gondii,in vitro,in vivo
نویسندگان مقاله قاسم عسگری | qasem asgari
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسین کشاورز | hossein keshavarz
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سعیده شجاعی | saeedeh shojaee
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد حسین معتضدیان | mohammad hossein motazedian
dept. of medical parasitology and mycology, faculty of medicine, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

مهدی محبعلی | mehdi mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رامین میری | ramin miri
medicinal and natural products chemistry research center, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

داود مهربانی | davood mehrabani
stem cell and transgenic technology research center, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

مصطفی رضاییان | mostafa rezaeian
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/482
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