این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۳۰، شماره ۲، صفحات ۴۵-۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Effect of Cold Atmospheric Plasma on Hsp90 Gene Expression in Terbinafine-Resistant Fungal Strains of the Trichophyton mentagrophytes Complex
چکیده انگلیسی مقاله
Introduction: The Trichophyton mentagrophytes complex, particularly terbinafine-resistant fungal strains, has emerged as a major cause of chronic and recurrent dermatophytosis, which is increasingly difficult to treat. These dermatophytes cause persistent infections of the skin, and nails, often accompanied by inflammation, severe itching, keratin degradation, and reduced quality of life. In fungi, the molecular chaperone Hsp90 is essential for stress tolerance, morphogenesis, and antifungal resistance. Cold atmospheric plasma (CAP), a non-thermal ionized gas, has recently gained attention for its notable antifungal activity. This study investigates the effect of CAP on Hsp90 gene expression in terbinafine-resistant isolates of the T. mentagrophytes complex.
Materials and Methods: Fifty isolates of the T. mentagrophytes complex were tested for terbinafine susceptibility using broth microdilution, following the CLSI M38-A2 protocol. Isolates with minimum inhibitory concentrations above 0.25 µg/mL were classified as resistant. These resistant isolates, along with a standard sensitive strain (PFCC 5809), were exposed to a helium-based CAP jet, which was applied directly to fungal suspensions in 96-well microtitier plates. The expression levels of Hsp90 before and after CAP exposure were quantified by real-time PCR using ΔΔCt method.
Results and Discussion: The baseline expression of Hsp90 was 124.7-fold higher in resistant isolates compared to the sensitive strain. CAP treatment reduced Hsp90 expression to 13.3-fold, which was significantly lower than baseline levels (p < 0.005).
Conclusion: CAP significantly downregulated Hsp90 expression in terbinafine-resistant fungal strains. These findings suggest that CAP may serve as a promising adjunct antifungal strategy to enhance the management of resistant dermatophytosis. 

 
""
کلیدواژه‌های انگلیسی مقاله Antifungal therapy, Cold atmospheric plasma, Hsp90, Terbinafine resistance, Trichophyton mentagrophytes
نویسندگان مقاله | Ameneh Shajari


| Zahra Salehi


| Mohammad Atyabi


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-907&slc_lang=other&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده other
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده کنگره
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات