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Iranian Journal of Basic Medical Sciences، جلد ۲۹، شماره ۴، صفحات ۵۹۸-۶۰۴
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عنوان انگلیسی Comparison of long non-coding RNA NEAT1 expression, P53, and antioxidant factors between Alzheimer’s patients and healthy individuals
چکیده انگلیسی مقاله Objective(s): Several studies have reported that the lncRNA Nuclear-enriched abundant transcript 1 (NEAT1) is associated with the progression of Alzheimer’s disease (AD). Oxidative stress and apoptosis also play crucial roles in the development of AD. The present study compared the serum levels of NEAT1, superoxide dismutase (SOD), glutathione (GSH), and P53 between individuals with AD and healthy controls. Materials and Methods: Peripheral blood samples were collected from 30 AD patients and 33 healthy controls and then centrifuged to separate serum. Total RNAs were isolated, and real-time polymerase chain reaction (RT-qPCR) was applied to determine NEAT1 gene expression. Also, Enzyme-linked immunoassays (ELISA) were conducted to measure SOD and GSH as anti-oxidant factors, and p53 as an apoptosis marker. Results: NEAT1 expression was significantly higher in AD patients than in controls (P<0.001). In addition, ROC analysis revealed that serum NEAT1 levels distinguished AD patients from healthy controls with 90% sensitivity and 84.85% specificity. Moreover, the anti-oxidant levels of SOD (P<0.0001) and GSH (P<0.05) were significantly decreased in AD patients, while P53 levels were significantly increased (P<0.05). However, no significant association was found between NEAT1 expression and the measured blood factors.Conclusion: The serum levels of NEAT1 effectively distinguished between AD patients and non-AD people, underscoring its potential as a blood-based biomarker for the development of Alzheimer’s disease.
کلیدواژه‌های انگلیسی مقاله Alzheimer disease RNA, Apoptosis, Long non-coding, NEAT1, Oxidative stress
نویسندگان مقاله | Sara Yarahmadi
Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran|Cellular and molecular research center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran


| Leila Alidoust
Department of Genetics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran


| Alia Saberi
Neuroscience Research Center, Trauma Institute, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran


| Behrooz Khakpour-Taleghani
Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran|Neuroscience Research Center, Trauma Institute, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran


| Mohamad Rostampour
Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran|Cellular and molecular research center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran


| Adele Jafari
Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_27441.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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