این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۹، شماره ۴، صفحات ۵۴۴-۵۵۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Characterization of exosomes from hypoxia-activated human amniotic membrane mesenchymal stem cells
چکیده انگلیسی مقاله Objective(s): Hypoxia is a physical stimulus that enhances stem cell activities to produce more cellular derivatives, particularly exosomes. Enhancing the quantity and quality of exosomes can improve their therapeutic properties. The study aimed to evaluate the effects of normoxic (22%O2) and hypoxic (1%O2) conditions on the characteristics of amniotic membrane-derived mesenchymal stem cells (AM-MSCs) and their exosomes.Materials and Methods: AM-MSCs were isolated, confirmed, and cultured under normoxic and hypoxic conditions. Exosomes were extracted from AM-MSCs and assessed for morphological characteristics (size/distribution/surface topography), structural properties (aggregation/colloidal particle behavior/surface charge/stability), chemical features (functional groups/ionic interactions), biological capacities (total protein concentration), and biocompatibility (microbiological quality/cytotoxicity/irritation/sensitization). Results: Hypoxia did not adversely affect the stemness potential of AM-MSCs (P>0.05). The average sizes of exosomes derived from AM-MSCs (AM-MSCs-Exo) were 185.7±23 nm (PI=0.756) and 145.4±36 nm (PI=0.420) under normoxic and hypoxic conditions, respectively (P≤0.05). Zeta potential of AM-MSCs-Exo was -12.57±0.5 mV under normoxia, while it was -2.37±0.73 mV in the hypoxic conditions. Exosomes from hypoxia-treated cells exhibited greater uniformity, dispersion, and stability than those from the normoxic group, resulting in reduced fluctuation under scattered light over time (P≤0.05). The total protein concentration in the hypoxic group was significantly higher than in the normoxic conditions (5.003 mg/ml vs. 4.109 mg/ml, representing a 1.22-fold increase) (P≤0.01). Exosomes extracted under normoxic and hypoxic conditions demonstrated acceptable biocompatibility with no signs of cytotoxicity, irritation, sensitivity, or microbial contamination.Conclusion: Hypoxic preconditioning enhances the yield and physicochemical stability of AM-MSC-derived exosomes, due to their unique composition and functional properties. 
کلیدواژه‌های انگلیسی مقاله Amniotic membrane, Biocompatibility, Extracellular vesicles, Hypoxia, Mesenchymal stem cells, Exosomes
نویسندگان مقاله | Hadiseh Kholgh
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Fateme Eshghabadi
Department of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran


| Hanieh Jafary
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Kavosh Zandsalimi
Department of Medical Laser (MLRC), Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran


| Nazanin Akbari
Department of Biology, Shahid Beheshti University, Tehran, Iran


| Azadeh Soltani
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Banafsheh Heidari
Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_27395.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات