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Iranian Journal of Basic Medical Sciences، جلد ۲۹، شماره ۲، صفحات ۲۶۱-۲۷۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Protocatechuic acid mitigates diazinon-induced lung injury in rats through modulation of oxidative stress, inflammatory, Keap-1/Nrf-2/HO-1 and ER stress-mediated apoptotic pathways |
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| چکیده انگلیسی مقاله |
Objective(s): Diazinon (DZN), a widely used organophosphate pesticide, induces pulmonary toxicity through oxidative stress, inflammation, endoplasmic reticulum (ER) stress, and apoptosis. This study investigated the potential protective effects of protocatechuic acid (PCA) against DZN-induced lung injury in rats.Materials and Methods: Thirty-five adult rats were randomly assigned to five groups (n = 7): Control, DZN (20 mg/kg), PCA100 (100 mg/kg), DZN + PCA50, and DZN + PCA100. Lung tissues were evaluated histopathologically, and oxidative stress markers (GSH, SOD, CAT, and GPx) and inflammatory mediators (TNF-α, IL-1β, IL-6, NF-κB, COX-2, and iNOS) were measured by ELISA. The protein levels of Keap-1, Nrf2, and HO-1 were assessed via Western blotting. Expression of ER stress–related genes (XBP-1, eIF2, ATF4, CHOP) and apoptotic markers (Bax, Bcl-2, caspase-3, -6, -9) was analyzed by qRT-PCR.Results: DZN exposure caused severe histopathological damage and significantly increased oxidative, inflammatory, ER stress, and apoptotic responses. PCA administration, particularly at 100 mg/kg, markedly improved lung morphology, normalized antioxidant enzyme levels, reduced cytokine production and NF-κB activation, and downregulated ER stress and apoptosis-related genes. PCA also enhanced Bcl-2 expression and activated the Nrf2/HO-1 signaling pathway.Conclusion: PCA exerts dose-dependent protective effects against DZN-induced pulmonary toxicity by modulating oxidative stress, inflammation, ER stress, and apoptosis. These findings suggest that PCA may serve as a promising therapeutic candidate for mitigating pesticide-related lung injury. |
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| کلیدواژههای انگلیسی مقاله |
Apoptosis, Diazinon, Endoplasmic reticulum - stress, Inflammation, Protocatechuic acid |
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| نویسندگان مقاله |
| Tuba Karaarslan
Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Türkiye
| Omercan Alat
Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Türkiye
| Ismail Can
Department of Histology-Embryology, Faculty of Medicine, Kafkas University, Kars, Türkiye
| Esra Aktas Senocak
Horasan Vocational College, Ataturk University, Erzurum, Türkiye
| Cihan Gur
Department of Medical Laboratory Techniques, Vocational School of Health Services, Atatürk University, Erzurum, Türkiye
| Ilyas Bozkurt
Department of Pharmacy Services, Nihat Delibalta Göle Vocational High School, Ardahan University, Ardahan, 75700, Türkiye
| Mohammad Alhilal
Department of Nursing, Faculty of Health Sciences, Mardin Artuklu University, Mardin, Türkiye
| Mesut Bunyami Halici
Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Türkiye
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| نشانی اینترنتی |
https://ijbms.mums.ac.ir/article_27258.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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