این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۷، شماره ۶، صفحات ۹۶۶-۹۷۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of Helicobacter pylori-derived outer membrane vesicles on the expression of inflammatory cytokines
چکیده انگلیسی مقاله Background and Objectives: Helicobacter pylori infection has been increasingly linked to extra-gastric diseases. Outer membrane vesicles are a key virulence factor of H. pylori. This study investigates the influence of H. pylori-derived outer membrane vesicles on inflammatory marker expression in human hepatoma cells (HepG2). Materials and Methods: Outer membrane vesicles were isolated through ultracentrifugation and characterized using dynamic light scattering technique (DLS) and a Field Emission Scanning Electron Microscope (FE-SEM). Protein concentrations were measured via the Bradford assay. HepG2 cells treated with outer membrane vesicles were analyzed for IL-6, TNF-α, TLR-4, TGF-β, and PPAR-γ mRNA expression by RT-qPCR. Cell viability was assessed through an MTT assay. The prevalence of H. pylori virulence-associated genes (babA2, sabA, and oipA) was determined by PCR. Results: The results showed a high prevalence of sabA (91.7%), babA2 (75%), and oipA (66.7%). FE-SEM and DLS analyses confirmed the presence of bleb-shaped nanovesicles ranging in size from 50 to 450 nm. H. pylori-derived outer membrane vesicles significantly upregulated the expression of pro-inflammatory markers (TLR-4, PPAR-γ, TNF-α, and IL-6), while downregulating TGF-β expression. Conclusion: These findings underscore the potential role of nanoparticles in driving inflammatory responses and influencing host cell signaling, which may play a key role in liver-related pathologies.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Maryam Mohammadbeigi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Amir Peymani
Department of Microbiology, Mazandaran University of Medical Sciences, Ramsar, Iran


| Shahin Bolori
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Simin Sotoudeh
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Rasoul Samimi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Anahita Bakhtiari
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Saina Shegefti
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mehdi Bakht
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Milad Badri
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Amir Javadi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Farhad Nikkhahi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/5253
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات