Introduction: Poaceous plants such as corn, wheat, barley, sorghum, oats and millet are an important part of agricultural ecosystems. Smuts are one of the most important fungal diseases of these plants, which often cause economic damage and the destruction of part or all of their yields. Materials and Methods: Poaceous smut infected plants in farms and pastures of Markazi and Lorestan provinces were sampled. Morphological characteristics of these fungi and their germination type of teliospores were studied with bright field and fluorescent microscopes and identified using valid keys. The phylogenetic relationship of these fungi with other smuts was also investigated based on ITS-rDNA region sequencing. Results: According to the type of host, morphological characteristics and mode of teliospores germination 12 species vs. U. maydis, U. hordei, U. turcomanica, U. avenae, U.nuda, U. bromivora, U. cynodontis, U. tritici, Tilletia laevis, Tilletia controversa Sporisorium reilianum and S. cruentum were diagnosed. The morphological characteristics and phylogenetic relationship of these fungi with other smuts are described. Conclusion: The smuts of poaceous plants in Markazi and Lorestan provinces include eight species of the genus Ustilago, two species of the genus Sporisorium and two species of the genus Tilletia.