| چکیده انگلیسی مقاله |
Background: A neglected zoonosis, cystic echinococcosis (CE) is the most common diseas in the developing nations worldwide. Vaccination is helpful in preventing the disease. Predicting main biochemical properties of the E. granulosus annexin (ANX) and its possible B-cell and human leukocyte antigen (HLA)-binding epitopes as a viable vaccine candidate was the goal of the current study. Methods: This study was done in Neyshabur University of Medical Sciences, Neyshabur, Iran. Predictions about transmembrane domain, subcellular localization, post-translational modification (PTM) sites, physico-chemical, antigenic, and allergenic profiles, secondary and 3D structure, tertiary model refinement, and validations were done using online servers. Results: The cytoplasmic 79.05 kDa protein was non-allergenic, hydrophilic (GRAVY: -0.490), stable (instability: 39.30), with improved thermotolerance (aliphatic: 80.07) and 122 post-translational modification sites. The secondary structure mostly included helices and extended strands. The 3D model was generated using Robetta server (confidence: 0.59) and was refined and validated subsequently. Shared B-cell epitopes were discovered using ElliPro, ABCpred and SVMTriP servers with antigenicity, allergenicity, and solubility screening. Moreover, multiple human and mouse MHC-binding epitopes were predicted and screened in E. granulosus ANX. Conclusion: This work offers a foundation for further investigation regarding designing an effective vaccination against CE. Further empirical research using examined protein alone or in conjunction with other antigens/epitopes is needed in the future. |
| نویسندگان مقاله |
| Afsaneh Khodadadi
Department of Pharmaceutical Sciences, Islamic Azad University, Tehran, Iran
| Mohammad Hosein Rastgoo
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
| Elaheh Ghiyasipour
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
| Reza Moradmandpaeinroudposhti
Department of Veterinary Science, Faculty of Veterinary Medicine, Islamic Azad University, Tehran, Iran
| Mohammad Arad Zandieh
Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran
| Fatemeh Zahirinia
Department of Veterinary Clinical Sciences, Urmia University, Urmia, Iran
| Mahdi Haghdadijoo
Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran
| Ali Esfandani
Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran
| Romina Rajabi
Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran
| Amirohssein Marashi
Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
| Mohammad Amin Dadboud
Faculty of Veterinary Medicine, Razi University of Kermanshah, Kermanshah, Iran
| Hamidreza Majidiani
Department of Basic Medical Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran
| Parham Rahmanian
Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran
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