این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Plant Physiology and Breeding، جلد ۱۵، شماره ۱، صفحات ۱۷-۳۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Parenchymal motor cells of pulvini base: An ideal explant for efficient direct regeneration in white poplar
چکیده انگلیسی مقاله Objective: Poplars (Populus sp.) are the most industrially important woody plants, garnering significant global attention for the introduction of new traits using genetic engineering and gene/genome editing approaches. However, the efficacy of genetic transformation and regeneration response of plant explants remains challenging among Populus species due to high variation in their genetic background. This work aimed to identify an optimal explant source to enhance high-frequency genetic transformation and genome editing capabilities in white poplar.
Methods: Transverse and longitudinal thin cell layer (t/lTCL) explants from Populus alba L. pulvinus and stem tissues were cultured on the shoot induction media with varying concentrations of BAP (0, 0.25, 0.5, 0.75, and 1.0 mg/L) in combination with IBA/NAA (0, 0.25, 0.5, and 0.75 mg/L) to evaluate their regeneration potential. The effect of node position on pulvini-tTCL regeneration was assessed, and histological analysis was performed on highly responsive explants. The root induction was examined using media with graded levels of IBA (0, 0.1, 0.2, 0.3 mg/L) and NAA (0, 0.05, 0.1, 0.15, and 0.2 mg/L).
Results: We obtained the fast and highly responsive tTCL-mediated shoot regeneration from the parenchyma cell layers (400-500 µm thickness) of the pulvinus basis of the 4th node cultured on shoot induction medium containing BAP 0.75 mg/L and IBA 0.5 mg/L, with a mean of 43.59 ± 0.22 shoots per explant. The regenerated shoots were well-rooted in root induction medium containing IBA 0.2 mg/L and NAA 0.1 mg/L. The well-hardened plants were transferred to greenhouse conditions.
Conclusion: The superior capacity of pulvini-tTCL tissue provides an opportunity to achieve high-frequency transgenic white poplar in genetic engineering and genome editing approaches.
کلیدواژه‌های انگلیسی مقاله Direct organogenesis,Microscopy analysis,Populus alba,Pulvinus,Thin cell layer,Tissue maturity culture,tTCL
نویسندگان مقاله Shahnoush Nayeri |
Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Bahram Baghban Kohnehrouz |
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, 51666, Iran.

نشانی اینترنتی https://breeding.tabrizu.ac.ir/article_20137_874ec88ff368ef4d02bfd4744d3344a1.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات