Bachground:Carbapanenems are β-lactam antibiotics that have multiple bactericidal activities. These antibacterial are usually considered to be the last-choice drugs in treating infections with multi-drug resistant Gram-negative bacteria.  Carbapenemase producing organisms (CPOs) are associated with serious health care-associated infections, making the mortality rate higher.

Methods:250 samples were collected with sterile cotton swabs; from wounds and burn wounds patients (140 wound swabs and 110 burn swabs). These swabs were cultured on MacConkey agar and cetrimide agar.  Cultures were incubated at 37°C for 18–24 hrs. The appearance of the colonies was evaluated by colour, shape, and overall morphology. BD Phoenix™ M50 device is used in identifying and making sensitivity test with BD RAPIDEC® CARBA NP assay in detecting carbapenemase enzymes.

Results:27(38.02%) isolates were carbapenemase producing and multi-drug resistance , distributed as following : 12 Carbapenemase producing Pseusomona aeruginosa multi-drug resistance, 9Carbapenemase producing Escherichia coli multi-drug resistance, 5carbapenemase producing Enterobacter cloacae multi-drug resistance and one isolate of Carbapenemase producing Klebsiella pneumoniae multi-drug resistance. The current study reported CP-Pseudomonas aeruginosa and CP-Klebsiella pneumoniae isolates as pathogens with the highest number of antibiotics resistance (resist 15 different antibiotics from seven classes of antibiotics).