| چکیده انگلیسی مقاله |
The success of spawning in striped catfish is largely determined by sperm quality, which can be influenced by several factors, including the age of the male broodstock. This study evaluates the influence of broodstock age on critical sperm quality metrics (motility, viability, and abnormalities) and reproductive success (fertilization and hatching rates) in striped catfish. For the treatments, male striped catfish were grouped into four age categories: Group A (1 year), Group B (2-4 years), Group C (5-7 years), and Group D (>8 years). Sperm volume was measured using a 10mL graduated tube, while sperm concentration, motility, viability, and abnormality were assessed microscopically using the Sperm Class Analyzer SCA® CASA system automatic sperm analyzer. To evaluate sperm performance during fertilization, eggs from the same female were used to assess fertilization rate, hatching rate, and larval abnormalities. The findings showed that male striped catfish aged 2-4 and 5-7 years demonstrated the most favorable sperm quality, with an average sperm volume of 51.0mL, concentration of 29.9million/mL, motility of 96.4%, viability of 97.4%, and abnormality rate of 3.6%. In contrast, the 1-year-old group showed the lowest performance, with an average sperm volume of 11.5mL, concentration of 27.7million/mL, motility of 66.5%, viability of 67.4%, and abnormality rate of 1.4%. Based on these results, it is recommended that broodstock aged 2-4 years be prioritized for spawning activities due to their higher sperm production, better sperm quality and they produce offspring that have good survival. |