این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Avicenna Journal of Phytomedicine، جلد ۱۵، شماره ۵، صفحات ۱۵۳۱-۱۵۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Green synthesis of selenium nanoparticles using Cordia myxa extract and assessment of their cytotoxic and antioxidant properties
چکیده انگلیسی مقاله Objective: This research aimed to explore the cytotoxic, apoptotic, and antioxidant effects of selenium nanoparticles (Se-NPs) produced by Cordia myxa (C. myxa) aqueous extract via a green reduction method.Materials and Methods: To synthesize Se-NPs, Na2SeO3 and C. myxa plant extract were used and their anticancer and antioxidant effects were investigated. After the synthesis of Se-NPs, their physicochemical properties were investigated by various techniques such as UV-Vis, XRD, TEM, FESEM/PSA, EDX, FT-IR, and DLS/Zeta. Next, the lethality of Se-NPs on cancerous Huh-7 and normal L929 cells was investigated. Then, using Annexin V/PI and DAPI kits, the number of apoptotic cells in terms of the obtained percentage and the amount of ROS production was determined by flow cytometry. The influence of Se-NPs on the expression of genes associated with apoptosis has been examined.Results: The XRD pattern and FESEM/TEM images confirmed the successful production of Se-NPs with an amorphous nature and size average of 11.9 nm. Flow cytometry analyses revealed a significant increase in ROS levels after 24 h of treatment with Se-NPs, demonstrating a concentration-dependent effect. Conclusion: The findings indicate that Se-NPs exhibit anticancer activity against Huh-7 cells, as evidenced by the upregulation of Bax, p53, and Caspase3 gene expression. Therefore, it can be asserted that Se-NPs have the potential to eliminate cancer cells, while simultaneously providing a protective effect on normal cells.
کلیدواژه‌های انگلیسی مقاله Nanoparticle, Cancer, Apoptosis, Biosynthesis, C. myxa plant
نویسندگان مقاله | Leili Hosseinpour
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Javad Baharara
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Majid Darroudi
Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Zahra Sabouri
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Maryam Haji Ghasem Kashani
Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran


| Saeed Zaker Bostanabad
Department of Biology, Parand Branch, Islamic Azad University, Tehran, Iran
نشانی اینترنتی https://ajp.mums.ac.ir/article_25954.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Research Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات