The water footprint is an analytical tool that offers a better and more comprehensive view of how consumers or producers engage with freshwater consumption. Given the water crisis in the country, particularly in Isfahan Province, this study aims to estimate and compare the direct and indirect water footprints of several large industries, including Mobarakeh Steel, Iron Smelting, Refinery, and Power Plant, located in the Zayandeh River Basin. After identifying the desired objectives and study areas, as well as confirming the availability of the required data, information was gathered from the selected industries. Two methods were then utilized to aggregate the entire chain and the sum of steps to calculate the water footprint in the researched industries. According to the calculations, the direct and indirect water footprint in the iron smelting industry amounts to 196.9 cubic meters per ton of steel annually, of which 4.026 cubic meters is attributed to direct consumption and 17.5 cubic meters to indirect consumption. In a refinery, 18.80 liters of water are consumed directly and indirectly to produce one barrel of product (gasoline or diesel). Additionally, the direct and indirect water footprint of the Islamabad power plant is 1,198,320 cubic meters per terajoule, equating to 4.31 liters per kilowatt hour. The results of this study indicate that the indirect water footprint in the analyzed industries is equal to or exceeds direct water consumption, with both being equally significant. Finally, it is important to note that the results of this study can support decision-makers and policymakers in the industry, including those in the iron and steel, refinery, and power plant sectors, in managing their water footprint.