Journal of Plant Physiology and Breeding، جلد ۱۴، شماره ۲، صفحات ۲۳-۳۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of mother-plant growing beds, explant type, and different disinfection treatments in control of Sansevieria rhizome contamination under in vitro conditions
چکیده انگلیسی مقاله Objective: Cloning ornamental plants, particularly chimeric varieties, is a crucial strategy for enhancing competitiveness in the ornamental plant market. Since the only method to propagate clones of chimeric Sansevieria varieties is through offset production from rhizome origins, optimizing sterilization techniques for in vitro rhizome explants is imperative. A significant drawback of plant tissue culture is the risk of microbial contamination, making the removal of contaminants a foundational step for successful tissue culture. Initial experiments on Sansevieria trifasciata rhizome explants revealed that conventional disinfectants were ineffective at eliminating contamination. Consequently, this study aimed to improve culture conditions by evaluating various factors essential for successful plant tissue culture.
Methods: In a preliminary experiment, two types of mother-plant growing media (perlite and conventional greenhouse soil) were compared using a t-test with 50 experimental units within a group. The second experiment was conducted on perlite as the mother-plant growing medium using a factorial arrangement with three factors based on the completely randomized design with three replications. The factors included three rhizome explant types (single node, terminal meristem, and rhizomes with terminal buds), benomyl and carbendazim fungicides at two concentrations of 2% and 10%, and mercuric chloride as the chemical disinfectant at two concentrations of 0.1% and 0.2% for 2 and 20 minutes plus a control without mercuric chloride.
Results: Results indicated that culturing mother plants in the perlite bed significantly reduced microbial loads. The results of the second experiment showed that treatment with 10% benomyl fungicide effectively diminished microbial contamination. Among the disinfectants tested, a 20-minute treatment of explants with 0.2% mercuric chloride resulted in the lowest fungal contamination. Among the explant types, single-node explants exhibited the least fungal contamination.
Conclusion: pre-treating single-node rhizome explants with 10% benomyl fungicide, followed by a 2-minute exposure to 70% alcohol and a 20-minute treatment with 0.2% mercuric chloride in a laminar flow cabinet, leads to optimal microbial decontamination. These findings underscore the importance of specific sterilization protocols in enhancing the efficacy of plant tissue culture methods for chimeric Sansevieria propagation.
کلیدواژه‌های انگلیسی مقاله Benomyl,Disinfection,Explant,Homogenous growth,Mercuric chloride,Rhizome,Tissue culture
نویسندگان مقاله Matin Kazemzadeh Bahnamirei |
Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Golestan, Iran

Mostafa K. Sarmast |
Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Mohammad Naghi Padasht Dahkaei |
Guilan Agricultural and Natural Resources Research and Education Center, AREEO, Rasht, Iran.

Mahdi Alizadeh |
Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

نشانی اینترنتی https://breeding.tabrizu.ac.ir/article_19313_1fa3c45a0f3cce84c78a692b96dd94b2.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات