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Iranian Journal of Biotechnology، جلد ۲۳، شماره ۳، صفحات ۴۲-۵۹
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عنوان انگلیسی Unlocking TB-COPD Pathogenesis: A Biotechnological Approach Targeting the PI3K/Akt Pathway
چکیده انگلیسی مقاله Background: Tuberculosis (TB)-associated chronic obstructive pulmonary disease (COPD) represents a significant global health challenge, yet its underlying molecular mechanisms remain poorly understood. The PI3K/Akt signaling pathway is implicated in inflammatory responses and cellular survival, suggesting its potential role in TB-COPD pathogenesis.

Objective: This study integrates computational and experimental biotechnology approaches to identify key molecular mechanisms linking TB and COPD, with a focus on the PI3K/Akt signaling pathway.

Methods: Weighted gene co-expression network analysis (WGCNA) was applied to two publicly available gene expression datasets (GSE42057, GSE83456) to identify differentially expressed genes and critical co-expression modules. Functional enrichment analysis (GO/KEGG) was performed to assess pathway involvement. Experimentally, a Mycobacterium tuberculosis-infected bronchial epithelial cell model (16HBE) was established, and the PI3K/Akt inhibitor LY294002 was utilized to evaluate its effects on apoptosis, proliferation, and epithelial-mesenchymal transition (EMT) markers.

Results: WGCNA identified a key module significantly enriched in PI3K/Akt signaling. Experimental validation demonstrated that LY294002 treatment significantly improved cell survival (p < 0.05), reduced apoptosis, and restored epithelial integrity, as indicated by increased E-Cadherin and decreased N-Cadherin expression. Furthermore, LY294002 suppressed PI3K/Akt mRNA expression and phosphorylation (p < 0.05), confirming pathway inhibition.

Conclusion: This study provides a novel biotechnological perspective on the role of PI3K/Akt signaling in TB-COPD, highlighting its potential as a therapeutic target. The integrative use of bioinformatics and experimental validation strengthens our understanding of molecular pathogenesis, paving the way for precision medicine strategies in respiratory disease management.
کلیدواژه‌های انگلیسی مقاله Biotechnology,PI3K/Akt signaling,Tuberculosis,Chronic Obstructive Pulmonary Disease,WGCNA,Molecular Pathway Analysis
نویسندگان مقاله Fengsen Li |
Fourth Clinical Medical College, Xinjiang Medical University

Jin Dai |
Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region; China

Zheng Li |
Xinjiang Respiratory Disease Research Laboratory, Urumqi 830000, Xinjiang Uygur Autonomous Region; China

Jing Jing |
Xinjiang Respiratory Disease Research Laboratory, Urumqi 830000, Xinjiang Uygur Autonomous Region; China

Dan Xu |
Xinjiang Respiratory Disease Research Laboratory, Urumqi 830000, Xinjiang Uygur Autonomous Region; China

Jing Wang |
Xinjiang Respiratory Disease Research Laboratory, Urumqi 830000, Xinjiang Uygur Autonomous Region; China

نشانی اینترنتی https://www.ijbiotech.com/article_224222_f8afdb5a319a9ad98ef8f5712964cda0.pdf
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