|
|
Cell Journal، جلد ۱۹، شماره supplement ۱، صفحات ۲۷-۳۶
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Expression of hsa-MIR-204, RUNX2, PPARγ, and BCL2 in Bone Marrow Derived Mesenchymal Stem Cells from Multiple Myeloma Patients and Normal Individuals |
|
| چکیده انگلیسی مقاله |
Objective: Multiple Myeloma (MM) is a heterogeneous cytogenetic disorder in which clonal plasma cells proliferate in the bone marrow (BM) and cause bone destruction. The BM microenvironment plays a crucial role in pathogenesis of this disease, and mesenchymal stem cells (MSCs) are one of the key players. Herein, we propose to investigate the expressions of hsa-MIR-204, runt-related transcription factor 2 (RUNX2), peroxisome proliferator-activated receptor gamma (PPARγ), and B-cell lymphoma 2 (BCL2) as factors involved in osteogenesis, adipogenesis, and MSC survival in BM-MSCs from MM patients and normal individuals.
Materials and Methods: In this experimental study, we isolated MSCs from BM aspirates of MM patients and healthy donors. Total RNA were extracted before and after co-culture with L363 myeloma cells. Gene expressions of RUNX2, PPARγ, BCL2, and hsa-MIR-204 were assessed by quantitive real time polymerase chain reaction (qRT-PCR).
Results: Higher levels of RUNX2, PPARγ, and hsa-MIR-204 expressions existed in MMMSCs compared to normally derived (ND)-MSCs. BCL2 expression decreased in MMMSCs. We observed different results in the co-culture model.
Conclusion: In general, the MM-MSCs gene expression profile differed compared to NDMSCs. Upregulation of RUNX2, PPARγ, and hsa-MIR-204 in MM-MSCs compared to NDMSCs would result in formation of bone defects. Downregulation of BCL2 would lead to MM-MSC cell death. |
|
| کلیدواژه‌های انگلیسی مقاله |
Multiple Myeloma,
Mesenchymal stem cells,
hsa-MIR-204,
RUNX2 |
|
| نویسندگان مقاله |
Raziyeh Mansurabadi |
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Saeid Abroun |
Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abass Hajifathali |
Bone Marrow Transplantation Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Amir Asri |
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Amir Atashi |
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mansoureh Haghighi |
Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical
Sciences, Iran
|
|
| نشانی اینترنتی |
https://www.celljournal.org/article_250931_c51e904acd0ea076faa8f2454e50da2b.pdf |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|