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Iranian Journal of Parasitology، جلد ۱۲، شماره ۱، صفحات ۵۶-۶۲
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی PCR-based Diagnosis of Toxoplasma Parasite in Ocular Infections Having Clinical Indications of Toxoplasmosis
چکیده انگلیسی مقاله Background: The diagnosis of ocular toxoplasmosis is mainly based on clinical features. However, ocular fluid testing by PCR may be very helpful for approval or rejection of this etiology. In this study, we utilized a nested-PCR technique, targeting the B1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the Toxoplasma DNA. Methods: Fifty aqueous or vitreous humor samples were obtained from patients with clinical features of ocular toxoplasmosis admitted to ophthalmology hospitals and clinics in Iran, within 2014. The samples were subsequently subjected to DNA extraction and purification. For nested amplification of the Toxoplasma B1 gene, two primer pairs were used. The outer and inner primers are expected to produce a 193 bp and a 96 bp fragments, respectively. Results: The first-round PCR resulted in the detection of T. gondii in 58% of samples by amplification of the expected 193bp DNA fragment. The nested-PCR using the inner primers, detected 15 additional samples from those with negative amplicons in the first round PCR (overall positivity of 88%). In addition, vitreous samples showed relatively more positive cases than aqueous humor in detection of the infection. Conclusion: The nested-PCR protocol using the B1 gene, with the high detection power, could be a useful complimentary method to clinical diagnose of ocular toxoplasmosis.
کلیدواژه‌های انگلیسی مقاله Ocular toxoplasmosis,Nested-PCR,Aqueous,Vitreous,Iran
نویسندگان مقاله عطیه فرهادی | atieh farhadi
dept. of parasitology and mycology, school of medicine, zanjan university of medical sciences, zanjan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)

علی هانیلو | ali haniloo
dept. of parasitology and mycology, school of medicine, zanjan university of medical sciences, zanjan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)

اصغر فضایلی | asghar fazaeli
dept. of parasitology and mycology, school of medicine, zanjan university of medical sciences, zanjan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)

سیامک مرادیان | siamak moradian
fellowship in vitreoretinal surgery, ophthalmic research center, labbafinejad medical center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی فرهادی | mehdi farhadi
dept. of parasitology and mycology, school of medicine, zanjan university of medical sciences, zanjan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1518
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349948.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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