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Iranian Journal of Parasitology، جلد ۱۲، شماره ۱، صفحات ۳۸-۴۵
عنوان فارسی
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عنوان انگلیسی Molecular Detection and Genotyping of Toxoplasma gondii in Chicken, Beef, and Lamb Meat Consumed in Northwestern Iran
چکیده انگلیسی مقاله Background: Toxoplasma gondii , the obligate, intracellular parasite afflicts human in diverse ways such as ingestion of tissue cysts in undercooked meat. The aim of this study was to assess the prevalence and genotyping of Toxoplasma isolated from meat samples being consumed in East Azerbaijan, Northwest of Iran. Methods: Overall, 150 samples including chicken, beef and lamb meat were collected from retailers in different regions of Tabriz, northwestern Iran during April to September 2014. Molecular detection was done by amplifying B1 gene and T. gondii surface antigen 2 (SAG2) loci. For genotyping of T. gondii, restriction fragment length polymorphism (RFLP) was performed using Sau 3AI and Hha I restriction enzymes. Finally, three positive isolate from each host was sequenced to evaluate and phylogenetic analysis. Results: Overall, 26 (17.33%) samples were positive for T. gondii including 4 (8%) isolate from chicken, 8 (16%) isolates from cattle and 14 (28%) isolates from sheep. According to the RFLP patterns, sequencing and phylogenetic results, all of the samples were identified as genotype I. Conclusion: The results indicated a potential risk of transmission of the disease through the consumption of infected meat. This is particularly important especially for pregnant women and immuno-compromised patients and this suggests that the training on the prevention of infection is necessary.
کلیدواژه‌های انگلیسی مقاله Toxoplasma gondii,Surface antigen P22,Meat,PCR,Iran
نویسندگان مقاله محمود محامی اسکویی | mahmoud mahami oskouei
immunology research center, tabriz university of medical sciences, tabriz, iran dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

محمدمرادی | mohammad moradi
immunology research center, tabriz university of medical sciences, tabriz, iran dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

اسماعیل فلاح | esmaeil fallah
dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

فایزه حمیدی | faezeh hamidi
dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نجیبه اصل رهنمای اکبری | najibeh asl rahnamaye akbari
dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1515
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349946.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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