|
|
Veterinary Research Forum، جلد ۸، شماره ۱، صفحات ۴۳-۴۸
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Changes in the expression of OCT4 in mouse ovary during estrous cycle |
|
| چکیده انگلیسی مقاله |
The transcriptional factor OCT4 regulates pluripotency of stem cells and has an important role during oocyte growth. Whereas, its role has remained ambiguous in ovarian tissue during reproductive cycle. Therefore, this study was aimed to investigate the expression patterns of OCT4 in mouse ovaries during the normal estrous cycle. Adult National Medical Research Institute mice were classified as proestrous, estrous, metestrous and diestrous on the basis of vaginal smear cytology. Their ovaries were removed and the protein and gene expression levels of OCT4 were assessed using immunohistochemical staining and real-time quantitative reverse-transcription PCR, respectively. Immunohistochemical staining revealed the expression of OCT4 in the cytoplasm of corpus luteum cells. In the follicles, OCT4 was expressed in the cytoplasm of granulosa cells. Furthermore, the gene expression levels of OCT4 was significantly higher in the proestrous phase than in the other phases of the estrous cycle (p < 0.05). The results indicated that OCT4 gene expression levels are affected by the cyclic pattern of the estrous cycle. |
|
| کلیدواژه‌های انگلیسی مقاله |
Estrous cycle,Expression,Mouse,OCT4,Ovarian tissue |
|
| نویسندگان مقاله |
نرگس باقری پور |
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)
سعید زواره |
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)
محمد تقی قربانیان | mohammad taghi
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)
سید حسن پای لاخی | seyed hassan
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)
سید رضا محبی | seyed reza
gastroenterology and liver diseases research center, research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
|
|
| نشانی اینترنتی |
http://vrf.iranjournals.ir/article_24317_69060c099bb107db84e0ac2c6c901db2.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/544/article-544-334253.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|