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مجله علوم اعصاب شفای خاتم، جلد ۲، شماره ۴، صفحات ۱۲۷-۱۲۷
عنوان فارسی P۷۷: Cell Therapy Approaches to Enhancing Neuro-Regeneration after Spinal Cord Injury: Generation Neural Stem Cells from Neurosphere-Derived Adipose Stem Cells Using Bioactive Substance TNT
چکیده فارسی مقاله لطفاً به چکیده انگلیسی مراجعه شود.
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عنوان انگلیسی P77: Cell Therapy Approaches to Enhancing Neuro-Regeneration after Spinal Cord Injury: Generation Neural Stem Cells from Neurosphere-Derived Adipose Stem Cells Using Bioactive Substance TNT
چکیده انگلیسی مقاله In the last few years, a lot of preclinical studies showed the therapeutic potential of stem cells in spinal cord injury (SCI). Neural stem cells (NSCs) can be differentiated into all cell types of spinal cord. NSCs are cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair of injury in central nervous system. Here in, we describe the efficient conversion of Adipose derived stem cells (ADSCs) into a neural stem cell-like under the influence of a factor inducing non-toxic bioactive substance TNT. ADSCs were isolated from adipose tissue of Wistar rat and were cultured. ADSCs were treated with bioactive substance TNT. These cells grew in neurosphere-like structures and differentiated to neural stem cells. Immunocytochemistry and RT-PCR techniques were performed to evaluate early neuroectodermal markers including SOX2, OCT4, NANOG, NeuroD and nestin. Our results showed that these markers expressed in high level. Also, NSCs were immunoreactive to NF68, NF200 and nestin. In addition, we confirmed the expression of SOX2, OCT4, NANOG and NeuroD genes by RT-PCR assay. The findings of this study provide a new method to generate NSCs from ADSCs by using non-toxic bioactive substance TNT, which can be helpful in cell therapy of SCI and degenerative diseases.
کلیدواژه‌های انگلیسی مقاله Neuroregeneration, Cell Therapy, Neurodegenerative Disease
نویسندگان مقاله prastoo براتی | prastoo barati
shefa neuroscience research center, khatam alanbia hospital, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

تقی طریحی | taghi tiraihi
a. shefa neuroscience research center, khatam alanbia hospital, tehran, iran. b. department of anatomy, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

مرضیه درویشی | marzieh darvishi
a. shefa neuroscience research center, khatam alanbia hospital, tehran, iran. b. department of anatomy, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

هادی کاظمی | hadi kazemi
shefa neuroscience research center, khatam alanbia hospital, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

نشانی اینترنتی http://shefayekhatam.ir/browse.php?a_code=A-10-24-475&slc_lang=fa&sid=fa
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/337/article-337-332324.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده تحقیقات پایه در علوم اعصاب
نوع مقاله منتشر شده پژوهشی
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