|
|
Iranian Journal of Chemistry and Chemical Engineering، جلد ۴۳، شماره ۳، صفحات ۱۳۰۲-۱۳۱۳
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Synthesis and Characterization of Acetamidotetrazole-Grafted Magnetic Chitosan Biopolymer as a Novel Non-Virus Vector for Targeted Gene Delivery into HECK-293T Cells |
|
| چکیده انگلیسی مقاله |
This study successfully synthesized a magnetic chitosan biopolymer covalently grafted onto acetamido tetrazole and evaluated it as a high-potential biocompatible non-viruses vector. The synthesized nanocarrier was fully characterized by FT-IR spectra, TEM and FESEM images, XRD, EDX, FESEM, and DLS analyses. The loading capacity of the plasmid was optimized with the ratio N/P 3 with excellent protection of the plasmid. The nanocarrier showed high gene expression and transfection in the HECK-293T cell line without any inherent toxicity. In addition, experimental results show that the new functionalized chitosan has excellent DNA condensation, protection, and high target transfection efficiency. Therefore, the novel magnetic chitosan nanocarrier functionalized with acetamido tetrazole has shown potential uses in cancer therapy. |
|
| کلیدواژه‌های انگلیسی مقاله |
Chitosan,5-amino-1H-tetrazole,gene delivery,gel retardation,MTT,VSM |
|
| نویسندگان مقاله |
Elaheh Mosaddegh |
Department of New Materials, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
Masoud Torkzadeh-Mahani |
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
Asadollah Hassankhani |
Department of New Materials, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
|
|
| نشانی اینترنتی |
https://ijcce.ac.ir/article_714734_844a88dde960d74e2cb54ee8f577fd4e.pdf |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|