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Medical Laboratory Journal، جلد ۱۰، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Chemiluminescence vs hemagglutination assay in the detection of anti-Treponema pallidum antibodies |
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| چکیده انگلیسی مقاله |
Background: Syphilis can have serious consequences on human health and poses a problem of biological diagnosis. Techniques such as chemiluminescence are still poorly known in our context. The objective was to compare chemiluminescence and hemagglutination assay with immunoblot as a reference test for the detection of anti-Treponema pallidum antibodies.
Methods: The detection of anti-Treponema pallidum antibodies was performed on serum. Comparison of chemiluminescence and hemagglutination assay was performed with immunoblot as the reference test on the basis of sensitivity, specificity, positive and negative predictive values and kappa statistic using IBM-SPSS version 24 software and the significance level was set at 5%.
Results: A total of 600 participants were recruited with a prevalence of syphilis infection of 3%. The sensitivity and specificity of chemiluminescence were 100% and 96.2% respectively, and 93.8% and 100% for hemagglutination assay respectively, Cohen's kappa statistic between the two tests showed satisfactory agreement (0.768). Cohen's kappa agreements between chemiluminescence/imunoblot and plate hemagglutination assay/imunoblot were greater than 0.8 indicating excellent agreement with the reference test (p<0.0001).
Conclusion: Chemiluminescence is more sensitive and less specific than hemagglutination assay. The interest of chemiluminescence as a first-line test for the diagnosis of syphilis should be considered. |
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| کلیدواژه‌های انگلیسی مقاله |
syphilis, chemiluminescence, hemagglutination assay, immunoblot |
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| نویسندگان مقاله |
| Toukam Michel
Department of Microbiology, Parasitology, Hematology and infectious diseases, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
| Voundi-Voundi Esther
Department of Microbiology, Parasitology, Hematology and infectious diseases, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
| Bole Bernard
Ministry of Public Health, Cameroon
| Mviena Nyimbe Louise
Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Cameroon
| Sahmo Simon Stevensen
Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Cameroon
| Nogue Raissa
Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Cameroon
| Kasia Jean Marie
Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
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| نشانی اینترنتی |
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1355-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
علوم آزمایشگاهی |
| نوع مقاله منتشر شده |
Research Article |
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