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Iranian Journal of Public Health، جلد ۵۳، شماره ۳، صفحات ۵۹۲-۶۰۴
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عنوان انگلیسی CircABCB10 Promotes the Apoptosis and Inflammatory Response of 16HBE Cells by Cigarette Smoke Extract by Targeting miR-130a/PTEN Axis
چکیده انگلیسی مقاله Background: Chronic obstructive pulmonary disease (COPD) has become a global public health problem due to its high mortality. So there is an urgent need to find an effective treatment Methods: The targeting relationship among circABCB10, miR-130a and PTEN was predicted by  the targetscan database. The lung condition was detected by CT(Computed Tomograph). The expression levels of circABCB10, miR-130a and PTEN in lung tissues were determined by qRT-PCR. The COPD model was established by stimulating normal and silenced 16HBE cells in circABCB10 genes with cigarette smoke extract (CSE) at different concentrations. qRT-PCR was conducted for the expression levels of circABCB10, miR-130a and PTEN, WB for the expression levels of apoptotic proteins,ELISA for the content of inflammatory factors,and CCK8 for the effect of CSE on the proliferation of cells. Results: CircABCB10 expression increased in lung tissues from patients with COPD and in 16HBE cells treated with CSE. The stimulation on cells with CSE increased the expression of inflammatory factors, while knocking down circABCB10 could reverse this response. The inflammatory response to the knockdown of circABCB10 was reversed by miR-130a inhibitor, which increased the expression of c-caspase 3. The targetscan database predicted the target factor downstream miR-130a was PTEN. Transfecting OE-PTEN reversed the inflammation of knocking down circABCB10, and increased the apoptosis and inflammation. Conclusion: CircABCB10 can cause the inflammatory response by targeting miR-130a/PTEN axis, which is a mechanism that may lead to the occurrence and development of COPD.
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نویسندگان مقاله | Changping Yun
Department of Respiration, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China


| Yuguang Wang
CT Room, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China


| Dongxu Wang
CT Room, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China


| Jialin Zang
CT Room, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China


| Zhen Lv
School of Pharmacy, Qiqihar Medical University, Qiqihar 161000, China


| Ruinan Liu
CT Room, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China


| Houyi Cong
CT Room, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/32079
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