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International journal of Advanced Biological and Biomedical Research، جلد ۲، شماره ۷، صفحات ۲۲۸۳-۲۲۸۷
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Study on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation |
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| چکیده انگلیسی مقاله |
Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme was stable in a range of pH 7.0–10.0 and the effect of the inhibitors showed it to be possibly an alkaline protease. Stability studies revealed temperatures around 40–60 ◦C. Results: The activity was reduced in the presence of Co2+, Fe2+ and Zn2+ ions, while the presence of K+ resulted in a discreet increase in proteolytic activity. The enzyme presented good stability towards organic solvents. Organic solvents such as 2-ethyl-1-hexanol and oleyl alcohol enhanced the activity of enzyme. The enzyme presented good stability towards oxidizing agent. The crude enzyme preparation was compatible with commercial detergents, retaining their 50–60% activities. Conclusions: The results demonstrated the importance of submerged fermentation for the production of protease. |
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| کلیدواژه‌های انگلیسی مقاله |
Protease,Submerged fermentation,Enzyme activity,Stabil |
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| نویسندگان مقاله |
بهزاد اطروشی |
department of microbiology, sciences and research branch, islamic azad university, rasht iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
معصومه انوری |
department of microbiology, faculty of sciences, islamic azad university, rasht branch, rasht, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
مهدی shariarinour |
department of microbiology, sciences and research branch, islamic azad university, rasht, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://www.ijabbr.com/article_8001_faeefbaae8eaf2b6a555b2b97d514998.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2255/article-2255-303193.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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