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Caspian Journal of Enviromental Sciences، جلد ۲۱، شماره ۵، صفحات ۱۱۵۱-۱۱۵۷
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عنوان انگلیسی Genetic and biological signature of Potato Virus X circulating in Kazakhstan
چکیده انگلیسی مقاله This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b2-7’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test.
کلیدواژه‌های انگلیسی مقاله Potato, Potato Virus X, isolates, ELISA, PCR, Targeted sequencing, Indicator plants
نویسندگان مقاله Moldir Azhimakhan |
Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan

Bibigul Beisembina |
Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan

Anastasiya Kapytina |
Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan

Nazym Kerimbek |
Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan

Dilyara Gritsenko |
Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan

Dianqiu Lv |
Southwest University, Chongqing, China

Hu Baigeng |
Leling Xisen Potato Industry Group Company Ltd, Leling, China

Vadim Khassanov |
Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan

نشانی اینترنتی https://cjes.guilan.ac.ir/article_7403_2c7039114b34460a9df55fcb3fa5c9e8.pdf
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