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Biomacromolecular Journal، جلد ۱، شماره ۱، صفحات ۶۹-۷۹
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| عنوان انگلیسی |
In Vitro Cytotoxic Activity and Binding Properties of Curcumin in the Presence of β-Casein Micelle Nanoparticles |
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| چکیده انگلیسی مقاله |
Curcumin (CUR) is the active curcuminoid with many physiological, biochemical, and pharmacological properties. Solubility and stability of CUR is the limiting factors for realizing its therapeutic potential. Bovine β-casein is an abundant milk protein that is highly amphiphilic and self-assembles into stable micellar nanoparticles in aqueous solution. β-Casein nanoparticle can solubilize CUR molecules. In the present study, we introduced a drug-delivery system comprising hydrophobic anticancer drug, CUR, entrapped within β-casein-based nanoparticles. The interaction of CUR with β-casein was investigated using steady-state fluorescence spectroscopy and molecular docking calculation. Results showed that at pH 7, CUR molecules bind to β-casein micelle and formed complexes through hydrophobic interactions. Förster energy transfer measurements and molecular docking studies suggested that CUR molecules bind to the hydrophobic core of β-casein. The binding parameters including number of substantive binding sites and the binding constant were evaluated by fluorescence quenching method. Additionally, the cytotoxicity of free CUR and CUR-β-casein complex to human breast cancer cell line MCF7 was evaluated in vitro. The study revealed that the CUR-β-casein complex exhibited better cytotoxic effects on MCF7 cells compared to equal dose of free CUR. |
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| نویسندگان مقاله |
فهیمه مهرانفر |
university of isfahan
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)
عبدالخالق بردبار | abdol khalegh
university of isfahan
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)
راضیه امیری امیری | razieh amiri
university of isfahan
سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)
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| نشانی اینترنتی |
http://www.bmmj.org/article_12730_15ab49ddee2db32fb8da0d0ce162cc97.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2124/article-2124-297435.pdf |
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| زبان مقاله منتشر شده |
en |
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