Veterinary Research Forum، جلد ۱۴، شماره ۱۲، صفحات ۶۷۳-۶۷۹
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عنوان انگلیسی How origin of ovaries influences the vitrification outcome of bovine ovarian tissue: effects of side of ovaries and corpus luteum
چکیده انگلیسی مقاله Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL˗ (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) In vitro culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries). The current study shows that the CL˗ and right groups had the greatest percentage of follicles with normal morphology compared to other vitrified-warmed groups. Furthermore, the levels of necrosis and tissue damage of the right cultured group were the lowest compared to other groups. It was shown that bovine ovarian tissues derived from right ovaries and ovaries without a corpus luteum can be functionally and morphologically preserved after vitrification. For the first time, the present study suggests that bovine ovarian tissue vitrification can be improved by considering the origin of the ovaries.
کلیدواژه‌های انگلیسی مقاله Corpus luteum, Cryopreservation, Immunolocalization, Ovarian tissue, Vitrification
نویسندگان مقاله Mohammadhamed Shahsavari |
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Gholamali Moghaddam |
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Kele Amaral Alves |
Department of Gynecology and Obstetrics, Medicine College, University of São Paulo, Ribeirão Preto, Brazil

Benner Geraldo Alves |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Laritza Ferreira De Lima |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Golshan Azimi |
Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

Deysi Jouana Dipaz Berrocal |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Lucina Mascena Silva |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Yago Pinto Da Silva |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Diego Alberto Montano Vizcarra |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

José Richardo De Figuereido |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

Ana Paula Ribeiro Rodrigues |
Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

نشانی اینترنتی https://vrf.iranjournals.ir/article_706634_da8b05dd678bdfe7d19c15c00d74f741.pdf
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