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Veterinary Research Forum، جلد ۱۲، شماره ۲، صفحات ۱۳۷-۱۴۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Viability of in vitro preserved sperm of Harris’s hawk (Parabuteo unicinctus) |
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| چکیده انگلیسی مقاله |
Harris hawks (Parabuteo unicinctus) are the raptors with more anthropogenic use, which makes it necessary to improve their reproduction either natural or assisted for sustainable use even though they are not endangered. The aim of this study was to determine fresh and thawed P. unicinctus sperm capacitation and acrosome reaction parameters as indicators of sperm viability in ejaculates of birds with injuries. A total of 30 ejaculates were analyzed as fresh and thawed samples. Basic sperm evaluations as well as in vitro acrosomal reaction capability through the presence and distribution of Ca2+ and N-acetylglucosamine and sialic acid using chlortetracycline and agglutinin-fluorescein isothiocyanate Triticum vulgaris were performed,respectively. Tagged samples were observed using epifluorescence microscopy. The results indicated 30.00% decrease in sperm viability following freeze-thaw, while rate of capacitated sperms (10.00%) and the percentage of sperm with acrosomal reaction (40.00%) were increased. The results of the experiment demonstrated that the post-thaw viability of the obtained sperm may be an alternative for use in artificial insemination. |
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| کلیدواژه‌های انگلیسی مقاله |
Acrosome,Cryopreservation,glucosamine,Membrane |
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| نویسندگان مقاله |
Cuauhtémoc Cruz-Valencia |
Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico
José Antonio Herrera-Barragán |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico
Fernando Gual-Sill |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico
Juán José Pérez-Rivero |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico
Zulma América Soto-Guerrero |
Secretary of Environment, Bird of Prey Rehabilitation Center, Aguascalientes, Mexico
Fernanda Rodriguez-Hernández |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico
Juán Gabriel Rivera-Martínez |
Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico
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| نشانی اینترنتی |
https://vrf.iranjournals.ir/article_243068_27837ed217f99f36779e0235de5c993b.pdf |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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