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Veterinary Research Forum، جلد ۱۳، شماره ۱، صفحات ۷-۱۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Investigation of the anticancer and apoptotic effect of Micromeria congesta under in vitro conditions and detection of related genes by real-time PCR |
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| چکیده انگلیسی مقاله |
At the present time cancer is one of the biggest health problems and because of the problems encountered in its treatment, alternative treatment methods of herbal origin are researched. In this study, the cytotoxic effects of the essential oil extracted from the Micromeria congesta plant on various cancer cells (A549, ECC-1, HCT-116, HELA, HGC-27, MDA-MB-231, SNU-423, U20S, DLD-1, PC-3) and normal cells (BEAS-2B, CRL-4010) have been examined. Anticancer mechanism of action has been particularly examined on gastric cancer (HGC-27; IC50: 15.84 µg mL-1), on which essential oil showed a high cytotoxic effect. In the study, the cytotoxic effect and the apoptotic effect have been applied by MTT and flow cytometric annexin-V methods, respectively. The apoptotic gene expression (caspase 3, caspase 9, MMP2, MMP9, ACTB) real-time PCR content analysis has been performed with gas chromatography mass spectrometry (GC-MS). M. congesta essentials oil has the highest cytotoxic effect on gastric cancer (HGC-27) cells, decreases MMP2 and MMP9 expressions, and induces apoptosis with increasing the expression of caspase 3 and caspase 8 genes. In addition, it has been determined that piperitenone oxide (40.00 - 45.00%), pulegone (11.00%) and cyclohexanone (18.00 - 19.00%) are the major components of M. congesta essentials oil. In conclusion, it has been determined that the compounds found in high amounts in M. congesta plant induces apoptosis by affecting the expression of compound genes and thus can have the potential to be an alternative drug in the treatment of gastric cancer. |
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| کلیدواژه‌های انگلیسی مقاله |
Apoptosis, HGC-27, Micromeria congesta, Real-time PCR |
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| نویسندگان مقاله |
Hikmet Dinç |
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkiye
Akın Yiğin |
Department of Genetics, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkiye
İsmail Koyuncu |
Department of Biochemistry, Faculty of Medicine, Harran University, Şanlıurfa, Turkiye
Mustafa Aslan |
Department of Educational Sciences, Faculty of Education, Harran University, Şanlıurfa, Turkiye
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| نشانی اینترنتی |
https://vrf.iranjournals.ir/article_248045_63d085c463c2ca8354ddfcae1fb233a9.pdf |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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