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Veterinary Research Forum، جلد ۱۴، شماره ۲، صفحات ۷۱-۷۷
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen |
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| چکیده انگلیسی مقاله |
The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The current study looks at the possible advantages of chilling canine sperm to 4.00 ˚C for three days using Tribulus terrestris aqueous extract (TTAE). Three mixed-breed dogs were utilized to extract 24 ejaculates, which were then diluted in a Tris-based extender. The ejaculates were then divided into five groups including 20.00, 40.00 and 50.00 µg mL-1 of TTAE, sham (distilled water devoid of TTAE) and control (without TTAE) groups. During the three days of experiment, several parameters were measured every 24 hr. It was noticed that after 48 and 72 hr of liquid storage, total and progressive motilities were greater in the group with the 40.00 µg mL-1 TTAE concentration than the control group. Compared to the control group, the group with the 40.00 µg mL-1 TTAE concentration exhibited superior motility and viability. The percentages obtained from the hypo-osmotic swelling test were much greater. In contrast to the control group, DNA integrity was poorer in the 40.00 µg mL-1 TTAE concentration. After 72 hr of storage, the group with 40.00 µg mL-1 TTAE concentration had lower malondialdehyde levels but considerably greater total anti-oxidant capacity, superoxide dismutase, glutathione peroxidase and catalase levels than the control groups. The current study found that supplementing the semen extender with 40.00 µg mL-1 TTAE improves semen parameters after 72 hr of storage at 4.00 ˚C, and therefore can improve fertilization efficiency. |
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| کلیدواژه‌های انگلیسی مقاله |
Anti-oxidant,Canine semen,Liquid storage,Tribulus terrestris aqueous extract |
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| نویسندگان مقاله |
Mohammad Moein Sabzeie |
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Esmail Ayen |
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ali Soleimanzadeh |
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mustafa Numan Bucak |
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
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| نشانی اینترنتی |
https://vrf.iranjournals.ir/article_701155_a591ab23d52657491be2d0e13184a8b0.pdf |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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