این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Physical Chemistry Research، جلد ۹، شماره ۳، صفحات ۴۲۷-۴۴۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Insights into Platinum Complex-HSA Bindings: Spectral and Molecular Dynamics Simulation Studies
چکیده انگلیسی مقاله Mutual interactions of human serum albumin (HSA) with the two binuclear platinum complexes, containing [(bhq)Pt(dppm)2(Cl)Pt(bhq)(Cl)] (1) and [(bhq)Pt(dppm)2(PhMe)Pt(bhq)(CO2CF3)] (2), in which bhq=benzo[h]quinoline, and dppm=bis(diphenylphosphino) methane, were thoroughly investigated using spectroscopic and molecular modeling techniques. In this respect, fluorescence, Ultraviolet-Visible (UV-Vis) and circular dichroism (CD) spectroscopies, along with the docking and molecular dynamics simulations (MD) were utilized.
Analysis of spectroscopic and MD simulation results revealed the structural alterations of HSA, upon binding to the binuclear platinum complexes, while the hydrogen bonding and van der Waals forces were found to mainly contribute to the protein-ligand intermolecular interactions. Results of far-UV CD measurements showed the strong ability of platinum complexes, in reducing the α-helical content of HSA, while other secondary structural features were increased.
Due to their different chemical natures, these complexes bind to HSA in different manners. Binding constants and thermodynamic binding parameters between these complexes and HSA were calculated using the Stern−Volmer and van’t Hoff equations. Calculated thermodynamic binding parameters indicated that the interaction is spontaneous and enthalpy driven, through the static and dynamic quenching mechanisms, for complexes 1 and 2, respectively.
کلیدواژه‌های انگلیسی مقاله Protein-ligand interaction,HSA,Platinum complex,Fluorescence spectroscopy,Molecular Dynamics Simulation
نویسندگان مقاله Abolfazl Ashrafi |
Department of Chemistry, Shiraz University of Technology, Shiraz 71555-313, Iran

Mohamad Mehdi Alavianmehr |
Department of Chemistry, Shiraz University of Technology, Shiraz 71555-313, Iran

Delara Mohammad-Aghaie |
Department of Chemistry, Shiraz University of Technology, Shiraz 71555-313, Iran

Reza Yousefi |
Protein Chemistry Laboratory (PCL), Department of Biology, Shiraz University, Shiraz 71454, Iran

Mohsen Golbon Haghighi |
Department of Chemistry, Shahid Beheshti University, Evin, Tehran 19839-69411, Iran

Ali Akbar Moosavi-Movahedi |
Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran

Navid Soltani Rad |
Department of Chemistry, Shiraz University of Technology, Shiraz 71555-313, Iran

نشانی اینترنتی https://www.physchemres.org/article_129261_ab40b98d16fe2a55320bca3e8032746f.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات