Iranian Journal of Chemistry and Chemical Engineering، جلد ۳۹، شماره ۶، صفحات ۲۸۱-۲۹۲
عنوان فارسی
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عنوان انگلیسی Aqueous Two-Phase Systems for the Isolation and Partial Purification of Lipases from Soil Bacteria
چکیده انگلیسی مقاله Aqueous Two-Phase System (ATPS) is an effective, simple, and single-step technique used for the partial purification of biological molecules. The aim of the study was to develop ATPS technique for the isolation and partial purification of lipases. ATPS composed of polyethylene glycol and ammonium sulfate was used in this study to partially purify lipase from the fermentation broth of a Bacillus strain isolated from local soil. The effect of different percent compositions of PEG 4000, 6000, and 10,000 along with 6.60%, 7.26%, 7.92%, and 8.26% ammonium sulfate was studied on the separation behavior of lipase. The optimal condition for the isolation and partial purification of lipases from soil bacterial was 12.5 % of PEG 10,000 with 7.92% ammonium sulfate. The recovery of lipase was 78.3% in the top phase (rich in PEG).
کلیدواژه‌های انگلیسی مقاله Aqueous two-phase system,Polyethylene glycol,ammonium sulfate,Lipases
نویسندگان مقاله Haris Saddique |
Department of Biotechnology, University of Malakand, Lower Dir, Khyber Pakhtunkhwa, PAKISTAN

Muhammad Aasim |
Department of Biotechnology, University of Malakand, Lower Dir, Khyber Pakhtunkhwa, PAKISTAN

Said Nawab |
Department of Biotechnology, University of Malakand, Lower Dir, Khyber Pakhtunkhwa, PAKISTAN

Noor Shad Bibi |
Department of Biochemistry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, PAKISTAN

Noor Muhammad |
Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, PAKISTAN

Muhammad Qasim |
Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, PAKISTAN

نشانی اینترنتی https://ijcce.ac.ir/article_36708_ca05d84a856e31292654c2f7fe033542.pdf
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