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Analytical and Bioanalytical Chemistry Research، جلد ۸، شماره ۳، صفحات ۲۷۵-۲۸۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Aptamer-based Electrochemical Detection of Tyrosinamide Using Metal-organic Frameworks/silver Nanoparticles Modified Glassy Carbon Electrode |
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| چکیده انگلیسی مقاله |
In this report, a new aptamer-based assay was presented reporting the electrochemical aptasensing for sensing tyrosinamide (Tyr-NH2). This strategy was relied on unbeatable conformational flexibility and specific recognition of aptamers. The tyrosinamide aptamer (Tyr-NH2-aptamer) was immobilized onto the metal-organic frameworks/silver nanoparticles modified glassy carbon electrode and hexacyanoferrate was selected as a probe to monitor interface variations during modification of the electrode and the aptamer conformational change generated by the Tyr-NH2 binding. Results showed that measurements by using electrochemical impedance spectroscopy had linear with the Tyr-NH2 concentrations in range of 0.01-0.25 nM and 0.25-1.15 nM. Detection limit of this system was found to be 2.3 pM. This method was also used to the Tyr-NH2 detection in serum samples successfully. Remarkable simplicity, ease of use and low-cost, make methodology as sensitive analytical system for sensing of the Tyr-NH2 that can be miniaturized. This strategy offers some promising advantages in reliable detection of the Tyr-NH2, which may be helpful in the routine analysis. |
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| کلیدواژه‌های انگلیسی مقاله |
Electrochemical aptasensor,Tyrosinaimde,Hexacyanoferrate,Metal-organic Frameworks,Silver nanoparticls |
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| نویسندگان مقاله |
Kazhal Ghanbari |
Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Mahmoud Roushani |
Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Azadeh Azadbakht |
Department of Chemistry, Khorramabad Branch Islamic Azad University Khorramabad, Iran
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| نشانی اینترنتی |
https://www.analchemres.org/article_130116_5a6fc42f8b669b6ee0ba4031e667c1d4.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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