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Analytical and Bioanalytical Chemistry Research، جلد ۱۱، شماره ۱، صفحات ۳۱-۳۹
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Hybrid Aptamer-Molecularly Imprinted Polymer: Synergistic Recognition and Ultrasensitive Detection of Helicobacter Pylori |
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| چکیده انگلیسی مقاله |
The combination of aptamer is performed with molecularly imprinted (MIP) using their unique features. In this sense, first the screen-printed carbon electrode (SPCE) was modified with nano size iridium cupper on nitrogen-doped carbon nanocapsoul (CN@IrCu) and, then, the Aptamer -MIP hybrid was used to detect Helicobacter pylori. This design leads to the use of dual properties of Aptamer and MIP. Moreover, CN@IrCu has been used as a substrate to increase aptamer loading and the conductivity. The sensing surface fabrication steps was measured by several different electrochemical techniques such as cyclic voltammetric (CV), differential pulse voltammetric (DPV), electrochemical impedance spectroscopy (EIS). The sensor shows a linear range from 102 CFU.ml-1 to 107 CFU.ml-1 with a detection limit of 33 CFU.ml-1. This Aptamer-MIP/CN@IrCu/SPCE was successfully evaluated in determining the concentration of Helicobacter pylori in blood serum samples with high reproducibility and selectivity. This MIP-aptasensor is low cost, low response time, high stability and good sensitivity. |
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| کلیدواژه‌های انگلیسی مقاله |
Molecularly imprinted polymers,Screen Printed Carbon Electrode,Aptamer,Helicobacter pylori,Aptamer-MIP |
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| نویسندگان مقاله |
Mahmoud Roushani |
Department of Chemistry, Ilam University, P. O. Box: 69315-516, Ilam, Iran
Masoumeh Sarabaegi |
Department of Chemistry, Ilam University, P. O. Box: 69315-516, Ilam, Iran
Hadi Hosseini |
Department of Chemistry, Ilam University, P. O. Box: 69315-516, Ilam, Iran
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| نشانی اینترنتی |
https://www.analchemres.org/article_182280_3385561015b2d2caead0e22cbc753cc9.pdf |
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| زبان مقاله منتشر شده |
en |
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