این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Tanaffos، جلد ۱، شماره ۳(summer)، صفحات ۳۵-۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Recovery of Mycobacteria from Clinical Specimens and Assessing Drug Susceptibility Test of Mycobacterium Tuberculosis Specimens by Mycobacteria Growth Indicator Tube (MGIT)
چکیده انگلیسی مقاله Background: Tuberculosis is a disease of global importance. Indeed, the lack of sensitive methods for the diagnosis and inappropriate therapy may lead to increased multidrug-resistance (MDR) cases. However, early detection and identification of acid fast bacilli (AFB) in clinical specimens can lead to effective intervention. Materials and Methods: The sputum specimens from 156 clinically suspected tuberculosis patients and 40 non- tuberculosis patients were digested, examined microscopically for acid- fast bacilli, and inoculated into “Mycobacterium Growth Indicator Tube” (MGIT), BACTEC –12B vial and onto Lowenstein- Jensen slants by standard procedures. Results: The result showed that smear was positive in 82(52.5%) and negative in 74 (47.5%) of 156 clinically suspected tuberculosis patients. The culture positive rate with Lowenstein- Jensen, MGIT, and BACTEC-12B vial were 122(78%), 136(87%), and 143(91%), respectively. Thereafter, MGIT indirect and direct susceptibility tests were performed on 15 sputum-positive specimens and the results were compared with proportional method. The results have revealed that accordance with proportional method was higher in MGIT indirect (83.5%) than direct (75%) susceptibility test, the difference was significant (p < 0.05). In another set of experiments, the indirect MGIT drug susceptibility test in 25 mycobacterium tuberculosis isolates were performed and compared with proportional method. The results showed that MGIT could correctly detect susceptibility to streptomycin, ethambutol, rifampin and isoniazid for 77.8%, 33%, 77.2% and 80%, respectively. Also, the agreement with proportional method for resistance were 88% for streptomycin, 80% for ethambutol, 80% for rifampin and 89% for isoniazid. Furthermore, by combining MGIT technology with L.J media, the mean time required for culture to grow for identification test was reduced from 22-28 to 12-16 days (p <0.05). Conclusion: MGIT is an efficient system to be used in center/ referral mycobacteriology laboratories of developing countries along with routine solid or liquid culture media. (Tanaffos 2002; 1(3): 35-44)
کلیدواژه‌های انگلیسی مقاله Mycobacterium tuberculosis,MGIT (Mycobacterium Growth Indicator Tube),Drug susceptibility test,Clinical specimen
نویسندگان مقاله Foroozan Mohammadi |
Department of Clinical Anatomical Pathology

Parisa Farnia |
Department of Mycobacteriology, NRITLD, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN- IRAN

Zohreh Mohammad-Taheri |
Department of Clinical Anatomical Pathology,

Abolhassan Zia- Zarifi |
Department of Mycobacteriology, NRITLD, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN- IRAN

نشانی اینترنتی https://www.tanaffosjournal.ir/article_241571_a7082010db5ac1ce80df9e061e87bff0.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات