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Tanaffos، جلد ۲، شماره ۱(winter)، صفحات ۲۹-۳۶
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عنوان انگلیسی The Results of Three Years Surveillance on Sputum Smear Microscopy in 285 District and Regional Tuberculosis Laboratories of Iran
چکیده انگلیسی مقاله Background: The study was designed to assess routine smear microscopy in 285 tuberculosis (TB) public health laboratories of Iran. Materials and Methods: Over one year period (1999), the information regarding infrastructure, specimen processing (smearing, staining, smear reading), and safety points of TB laboratories were collected. Thereafter, in two consecutive years (2000 & 2001), the accuracy of smear reading was investigated by sending a set of 6 blinded slides to each laboratory. In total, 1710(X2) slides were prepared, of which 855 were positive (having varying degree of positivity), and 855 were negative. Results: We found that 68.4% of TB laboratories were using commercially prepared staining kits of inferior quality and 72% of TB technicians examined, each slide for less than 7 minutes whereas the recommended standard time is 15-20 minutes. The results of blinded smear reading in the first round demonstrated 71% of accuracy. However, after 3 days of smear microscopy training for personnel in 60 poorly performing laboratories, we observed a substantial improvement in the quality of microscopy. The number of false positive reports dropped from 20 to 0 (p <0.05), and the number of negative reports declined from 40 to 5 (p <0.05). Overall, out of 237 participating laboratories in the second round, 217(91%) laboratories could correctly detect positive and negative smears. Conclusion: This study demonstrates the importance of quality control and correct performance of smear microscopy. It also emphasizes the need for implementing strict and ongoing quality control for all laboratory procedures. (Tanaffos 2003; 2(5): 29-36)
کلیدواژه‌های انگلیسی مقاله Smear Microscopy,TB Laboratory,quality control
نویسندگان مقاله Parissa Farnia |
Department of Mycobacteriology

Mohammad Reza Masjedi |
Department of Pulmonary Medicine

Foroozan Mohammadi |
Department of Clinical Anatomical Pathology, NRITLD, Shaheed Beheshti University of Medical Sciences,

Mahshid Nasehi |
Center for Control and Preventive Diseases

Mokhtar Foroozesh |
National Reference Laboratories, Institute Pasteur of IRAN,

Salek Salek |
Center for Control and Preventive Diseases,

Mohammad Abbasi |
National Reference Laboratories, Institute Pasteur of IRAN,

Ahmad Reza Bahrmand |
National Reference Laboratories, Institute Pasteur of IRAN,

Kiumars Ghazisaidi |
Department of Mycobacteriology,

Mohammad Feyzabadi |
National Reference Laboratories, Institute Pasteur of IRAN,

Abol-Hassan Zia Zarif |
Department of Mycobacteriology,

Moslem Bahadori |
Department of Clinical Anatomical Pathology, NRITLD, Shaheed Beheshti University of Medical Sciences,

Ali Akbar Velayati |
Department of Pediatrics, NRITLD, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN-IRAN

نشانی اینترنتی https://www.tanaffosjournal.ir/article_241552_4b5952c44fbd7e1e0024dc49dfa6a0af.pdf
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