این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Tanaffos، جلد ۱۵، شماره ۳، صفحات ۱۴۷-۱۵۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی nsights into Pyrazinamidase and DNA Gyrase Protein Structures in Resistant and Susceptible Clinical Isolates of Mycobacterium tuberculosis
چکیده انگلیسی مقاله Background: Mutations in pncA and gyrA genes cause pyrazinamide (PZA) and fluroquinolone resistance in Mycobacterium tuberculosis (MTB). In the present study, structures of pyrazinamidase (PZase) and DNA gyrase proteins were studied in resistant and susceptible clinical isolates of MTB. Materials and Methods: Sixty clinical isolates of MTB were used in this study. Polymerase chain reaction (PCR) amplification of pncA and gyrA genes was accomplished on purified DNA. Sequence of the fragments was determined by an Applied Biosystems TM apparatus. Bioinformatic analysis was performed by online software and three-dimensional (3D) structures of proteins was predicted using Molegro Virtual Docker (MVD) Modeler software. Results: Amplified 744 and 194 bp fragments of pncA and gyrA genes, respectively were yielded suitable sequence results. Predicted 3D structures of proteins showed some differences between wild-type and mutant structures. Mutation in amino acid No.31 (T92C) caused an increase in distance from metal ion position to enzyme active site, but it was considered as a polymorphism. Docking results by MVD revealed a relationship in quinolone resistancedetermining regions (QRDR) amino acids in interaction with antibiotic. T92C mutation in PZase from non-polar aliphatic amino acid Ile (ATC) to polar aliphatic amino acid threonine (ACC) was a polymorphism. Conclusion: Structural changes in two important proteins related to drug resistance were proven in clinical isolates of MTB.
کلیدواژه‌های انگلیسی مقاله Mycobacterium tuberculosis,Pyrazinamidase,DNA Gyrase,Protein
نویسندگان مقاله Azam Ahmadi |
Department of Molecular Genetics, Tarbiat Modares University,

Raziyeh Nazari |
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran,

Mohammad Arjomandzadegan |
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran,

Mohammad Reza Zolfaghari |
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran,

Vahideh Vahidi |
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran,

Toktam Poolad |
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran,

Manijeh Kahbazi |
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran,

Maryam Sadrnia |
Department of Biology, Payame Noor University, I.R. of Iran,

Mojtaba Tousheh |
Department of Cellular and Molecular Medicine, Isfahan University, Isfahan, Iran

Pourya Rafiee |
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran,

نشانی اینترنتی https://www.tanaffosjournal.ir/article_240218_6257688657a48d01ac2db6777abc8be8.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات