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International journal of Advanced Biological and Biomedical Research، جلد ۱۱، شماره ۳، صفحات ۱۱۵-۱۲۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Investigating the Expression Levels of Glutathione Peroxidase and Glutathione Reductase Genes in Mastectomies Women |
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| چکیده انگلیسی مقاله |
Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.
Procedure: After cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a CO2 incubator at 37°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.
Results: The concentration of 500 micrograms of nanoparticles caused the death of more than 60% of MCF 7 cells (P< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal HEK293 cell line .
Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-7 breast cancer cell line of mastectomy candidates.
Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.
Procedure: After cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a CO2 incubator at 37°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.
Results: The concentration of 500 micrograms of nanoparticles caused the death of more than 60% of MCF 7 cells (P< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal HEK293 cell line .
Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-7 breast cancer cell line of mastectomy candidates. |
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| کلیدواژه‌های انگلیسی مقاله |
glutathione peroxidase,
Glutathione,
Mastectomy,
Breast cancer,
Gene expression |
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| نویسندگان مقاله |
Parmiss Adyani Kalvanagh |
Postgraduate Student, experimental sciences, Tabriz, Iran
Yousef Adyani Kalvanagh |
Breast Surgery Fellowship, Tabriz University of Medical Sciences, Tabriz, Iran
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| نشانی اینترنتی |
https://www.ijabbr.com/article_705784_1750b427e1c784c159920bb9890f12fc.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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