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Biomacromolecular Journal، جلد ۷، شماره ۳، صفحات ۱۵۹-۱۶۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Investigation of Activity, Stability, and Structural Changes of Aspergillus Oryzae α-Amylase in the Presence of Water 1-Ethyl-3-methylimidazolium Acetate Mixture |
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| چکیده انگلیسی مقاله |
α-Amylases catalyze the hydrolysis of starch and are widely used in various industries, especially in the food industry. Despite the high demand for the use of α-amylase in various industries, most α-amylases do not have the temperature and pH stabilities for use in industrial processes. Ionic liquids are broadly used as co-solvents to improve the activity, stability, and selectivity of enzymes. In this study, the effect of 1-Ethyl-3-methylimidazolium Acetate on the activity, temperature and pH optimum, thermal stability, and structure of an α-amylase from Aspergillus oryzae were investigated. The activity of α-amylase was decreased with increments in [EMIm][Ac] concentration. Vm of α-amylase in the presence of [EMIm][Ac] was increased while Km was decreased. Same as the absence of [EMIm][Ac], the optimal pH and temperature in the presence of [EMIm][Ac] were 7.5 and 45 °C, respectively. The inactivation rate of α-amylase at temperatures of 40, 50, and 60 °C in the presence of both 0.4 and 1 M [EMIm][Ac] is less than in its absence. Intrinsic fluorescence spectroscopy revealed structural changes in the presence of [EMIm][Ac]. These results indicate the improvements in the activity, optimum temperature, and thermal stability of α-amylase due to the structural changes induced with [EMIm][Ac]. |
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| کلیدواژه‌های انگلیسی مقاله |
Keywords Ionic liquids,
[EMIm][Ac],
Fluorescence spectroscopy |
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| نویسندگان مقاله |
Reza Farahmand |
Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran
Mehdi Ebrahimi |
Department of Biochemistry and Biophysics, College of Biological Sciences, Varamin-Pishva Branch,
Islamic Azad University, Pishva, Iran
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| نشانی اینترنتی |
https://www.bmmj.org/article_703833_bb71cf348ef0a072d88fc3cdb16e532a.pdf |
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| زبان مقاله منتشر شده |
en |
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