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Iranian Journal of Parasitology، جلد ۱۸، شماره ۴، صفحات ۵۴۶-۵۵۳
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عنوان انگلیسی Gelatin Zymography of Major Proteases in Exosomes of Leishmania major Promastigotes
چکیده انگلیسی مقاله Background: Enzymatic digestion of extra cellular matrix proteins by proteinases of   Leishmania promastigotes is a complex process. Hence, studies on functional proteomics of these enzymes can help select these enzymes as possible vaccine candidates or selecting candidates for chemotherapy and immunotherapy. Several proteolytic enzymes are involved in virulence of Leishmania spp. These enzymes are mostly serine, cysteine and metalloproteases. We aimed to detect proteases in Leishmania promastigote exosomes. Methods: Serine, cysteine and metalloproteases were investigated in exosomes and lysate of L. major promastigote using gelatin zymography. The study was carried out in the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, in 2021. Results: Zymography findings of metalloproteinases showed transparent bands, including a 63-kDa glycoprotein (GP63). This glycoprotein is a major surface metalloproteinase. In addition, transparent bands belonged to serin proteases and cathepsin were demonstrated in gels associated to Leishmania promastigote lysate and exosomes. Conclusion: Several metalloproteases, serin proteases and cathepsins were shown in promastigote lysate and exosomes of L. major, which could purified and used as fractions for immunodiagnostic.
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نویسندگان مقاله | Akram Azambakhtiar
Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Sedigheh Nabian
Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Mehdi Mohebali
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Taheri
Rastegar Reference Laboratory, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Ramin Mazaheri Nezhad Fard
1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3653
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