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Veterinary Research Forum، جلد ۷، شماره ۴، صفحات ۲۸۷-۲۹۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Molecular identification and phylogenetic analysis of Lactobacillus and Bifidobacterium spp. isolated from gut of honeybees (Apis mellifera) from West Azerbaijan, Iran |
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| چکیده انگلیسی مقاله |
Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and phylogenetic analysis were used for molecular identification of lactic acid bacteria (LABs) isolated from Apis mellifera. Eighteen honeybee workers were collected from three different apiaries in West Azerbaijan. LABs from the gut of honeybees were isolated and cultured using routine biochemical procedures. Genomic DNA was extracted from LABs and a fragment of 1540 bp in size of 16S rRNA gene was amplified. PCR products were digested using HinfI endonuclease and digested products with different RFLP patterns were subjected to nucleotide sequencing and phylogenetic analysis. The results revealed that Lactobacillus and Bifidobacteria spp. are were the most abundant LABs in honeybee gut. Phylogenetic analysis showed that both Lactobacillus and Bifidobacterium were closely clustered with high similarity percentage with the same bacteria isolated from honeybees’ gut elsewhere. It was concluded that LABs isolated from honeybees had low sequence divergence in comparison with LABs isolated from other sources such as dairy products. |
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| نویسندگان مقاله |
محمد فاروق شریف پور | mohammad farouq
private veterinary practitioner, mahabad, iran
کریم مردانی |
department of food hygiene and quality control, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)
عبدالغفار اونق |
department of microbiology, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)
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| نشانی اینترنتی |
http://vrf.iranjournals.ir/article_22164_0d1b76c6a946ff87a0270c4d2562ef1b.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/544/article-544-279624.pdf |
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| زبان مقاله منتشر شده |
en |
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