|
|
Infection Epidemiology and Microbiology، جلد ۹، شماره ۱، صفحات ۰-۰
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Evaluation of the Relative Frequency of Carbapenemase Genes by Phenotypic and Genotypic Methods in Pseudomonas aeruginosa Isolates from Patients with Open Heart Surgery in Iran |
|
| چکیده انگلیسی مقاله |
Aims: Carbapenem resistance among Pseudomonas aeruginosa strains is alarming. This study aimed to investigate the relative frequency of carbapenem-resistant P. aeruginosa strains by phenotypic and genotypic methods. Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR). Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC> 8 µg/mL for imipenem and meropenem), E-test (MIC ˂32 µg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates. Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%). |
|
| کلیدواژه‌های انگلیسی مقاله |
Carbapenemases, Pseudomonas aeruginosa, Phenotypic, Bacterial sensitivity tests |
|
| نویسندگان مقاله |
| Maryam Mokhtari
Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| Ali Mojtahedi
Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| Nejat Mahdieh
Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
| Alireza Jafari
Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| Zahra Atrkar Roushan
School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
| Mohammad Javad Arya
Sina Pathobiology lab, Yazd, Iran
|
|
| نشانی اینترنتی |
http://iem.modares.ac.ir/browse.php?a_code=A-10-74190-1&slc_lang=en&sid=4 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|