Iranian J. of Fisheries Science، جلد ۲۱، شماره ۵، صفحات ۱۲۷۸-۱۲۹۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Bioinformatics and mRNA expression of catalase gene and determination of catalase enzyme activity in zebrafish (Danio rerio) exposed to the herbicide paraquat
چکیده انگلیسی مقاله Effects of two different doses, treatment I (0.30 ppm) and treatment II (0.15 ppm) of paraquat, were investigated on mRNA transcript of the catalase gene (cat) and CAT activity in zebrafish. We determined tissue-specific distribution of zebrafish cat and bioinformatics analysis. Muscle, liver, intestine, heart, kidney, brain, eye, gills, swim bladder, skin, ovary and testis tissue samples from three female and three male fish were dissected to detect tissue-spesific distrubition while liver and gill tissues were dissected to evaluate the effect of acute stress in zebrafish. The steady-state level of zebrafish cat transcript in gill (311±11.32) was lower than liver (595.53±14.41) but higher than muscle (132.89±9.21), testis (120.03±6.99), kidney (118.96±8.87), ovary (104.31±8.22), intestine (37.28±3.11), brain (19.17±2.01), eye (18.30±2.10), heart (15.79±2.01), spleen (7.7±0.91), swim bladder (6.97±0.21), and skin (4.59±0.31). Expression of cat was significantly decreased in liver and gill tissues after exposure to two concentrations of paraquat (p< 0.05). Measurement of enzyme activities in zebrafish, exposed to 0.30 and 0.15 ppm of paraquat for 72 h duration, showed significant (p< 0.05) variations in CAT activity compared to that of the control. Different steady-state levels among tissues in zebrafish could be explained by duplicated genes in teleost fish that arose through teleost specific-whole genome duplication exhibiting a dissimilar tissue distribution.
کلیدواژه‌های انگلیسی مقاله Bioinformatics, Gene expression, Enzyme activity, catalase, Zebrafish
نویسندگان مقاله M. Bayır |
Department of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.

E.C. Çapan |
Department of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.

S. Keşan |
Department of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.

نشانی اینترنتی https://jifro.areeo.ac.ir/article_128011_5b52ca787450d416e44f0fcebef6b14c.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات